Protein Modification Reagents
Thermo Scientific™ DTNB Pierce™ (reagente di Ellman)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Thermo Scientific™ Cisteina·HCl Pierce™
Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).
Thermo Scientific™ Papaina immobilizzata Pierce™
Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.
Thermo Scientific™ Pepsina immobilizzata Pierce™
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Gel a TCEP immobilizzato riducente il disolfuro Pierce™
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific™ Urea
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Proteinasi K Pierce™
Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.
Thermo Scientific™ Soluzione TCEP a pH neutro Bond-Breaker™
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ Soluzione di guanidina·HCl Pierce™ 8M
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Thermo Scientific™ Pierce™ TCEP·HCl
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Thermo Scientific™ N-etilmaleimide (NEM) Pierce™
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Thermo Scientific™ ™2-mercaptoetanolo Pierce
Beta-mercaptoetanolo (bME, 2BME, 2-ME, b-mer), CAS 60-24-2 puro, liquido (equivalente a 14 M); agente riducente con tiolo per la scissione dei ponti disolfuro proteici (cistine, cisteine).